ALKBH3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALKBH3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12292-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12292-1-AP (same clone as 12292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12292-1-AP (same clone as 12292-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 12292-1-AP (same clone as 12292-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12292-1-AP (same clone as 12292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12292-1-PBS in a different storage buffer formulation.

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Product Information

12292-1-PBS targets ALKBH3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH3 fusion protein Ag2982
• Full Name: alkB, alkylation repair homolog 3 (E. coli)
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 22-25 kDa, 33kDa
• GenBank Accession Number: BC015155
• Gene Symbol: ALKBH3
• Gene ID (NCBI): 221120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.