ALKBH3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ALKBH3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12292-1-AP

Synonyms

ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1

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Validation Data Gallery

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  WB analysis using 12292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12292-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12292-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human lung cancer tissue, human testis tissue, human prostate cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

12292-1-AP targets ALKBH3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALKBH3 fusion protein Ag2982
• Full Name: alkB, alkylation repair homolog 3 (E. coli)
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 22-25 kDa, 33kDa
• GenBank Accession Number: BC015155
• Gene Symbol: ALKBH3
• Gene ID (NCBI): 221120
• RRID: AB_11125161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

Publications

Species	Application	Title
human	WB,IHC	J Gastroenterol Hepatol; ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.; Authors - Qian Wang ; KD Validated
human	WB	Cell Biosci; m1A demethylase Alkbh3 regulates neurogenesis through m1A demethylation of Mmp15 mRNA; Authors - Huan Wang
human	IHC	Biomedicines; The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis; Authors - Claudia Alexandra Dumitru
human	IHC	Clin Transl Med; PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression; Authors - Yongxia Chang