ALDH2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ALDH2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G10F4

Cat no : 68237-1-PBS

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

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Validation Data Gallery

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  WB analysis of mouse liver using 68237-1-Ig (same clone as 68237-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68237-1-Ig (same clone as 68237-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68237-1-Ig (same clone as 68237-1-PBS)

  WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit liver using 68237-1-Ig (same clone as 68237-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 68237-1-Ig (same clone as 68237-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68237-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68237-1-PBS targets ALDH2 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH2 fusion protein Ag7463
• Full Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002967
• Gene Symbol: ALDH2
• Gene ID (NCBI): 217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.