ALDH2 Monoclonal antibody

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  WB analysis of mouse liver using 68237-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68237-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68237-1-Ig

  WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells.

• 

  WB analysis of rabbit liver using 68237-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68237-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

ALDH2 Monoclonal Antibody for WB, ELISA

Cat No. 68237-1-Ig

Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig and More (1)

Applications

WB, ELISA

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: mouse liver tissue, rabbit liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

68237-1-Ig targets ALDH2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH2 fusion protein Ag7463
• Full Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002967
• Gene Symbol: ALDH2
• Gene ID (NCBI): 217
• RRID: AB_2935324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05091
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Publications

Species	Application	Title
mouse	WB	Arch Biochem Biophys; Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice; Authors - Na Lu
mouse	WB	Mol Nutr Food Res; Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice; Authors - Meng-Yuan Liu