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ALDH2 Monoclonal antibody

ALDH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G10F4

Cat no : 68237-1-Ig

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of mouse liver using 68237-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of pig liver using 68237-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of HepG2 using 68237-1-Ig

    WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells.

  • Western Blot (WB) analysis of rabbit liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of rabbit liver using 68237-1-Ig

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using ALDH2 Monoclonal antibody (68237-1-Ig)

    WB analysis of rat liver using 68237-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, rabbit liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68237-1-Ig targets ALDH2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ALDH2 fusion protein Ag7463
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated Molecular Weight 56 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC002967
Gene Symbol ALDH2
Gene ID (NCBI) 217
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Protocols

Product Specific Protocols
WB protocol for ALDH2 antibody 68237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Arch Biochem Biophys

Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice

Authors - Na Lu
View Article