ALDH1A1 Recombinant antibody, PBS Only (Detector)

ALDH1A1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240995F4

Cat no : 83830-2-PBS

Synonyms

ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase

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Validation Data Gallery

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  WB analysis using 83830-2-RR (same clone as 83830-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83830-2-RR (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83830-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00795-2

  Sandwich ELISA standard curve of MP00795-2, Human ALDH1A1 Recombinant Matched Antibody Pair - PBS only. 83830-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8665. 83830-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

• 

  Sample test of MP00795-2

  The mean ALDH1A1 concentration was determined to be 3.58 ng/mL in A549 cell extract based on a 1.00 mg/mL extract load and 2.49 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load.

• 

  Sample test of MP00795-2

  Serum of eight individual healthy human donors was measured. The ALDH1A1 concentration of detected samples was determined to be 0.25 ng/mL with a range of 0.01-0.66 ng/mL.

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  IHC staining of human cervical squamous cancer using 83830-2-RR (same clone as 83830-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83830-2-RR (ALDH1A1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83830-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00795-1

  Cytometric bead array standard curve of MP00795-1, ALDH1A1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83830-1-PBS. Detection antibody: 83830-2-PBS. Standard: Ag8665. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 83830-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83830-2-RR against Human ALDH1A1 were performed. The affinity constant is 0.322 nM.

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Product Information

83830-2-PBS targets ALDH1A1 as part of a matched antibody pair:

MP00795-1: 83830-1-PBS capture and 83830-2-PBS detection (validated in Cytometric bead array)

MP00795-2: 83830-3-PBS capture and 83830-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALDH1A1 fusion protein Ag8665
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).