ALDH1A1 Recombinant antibody

ALDH1A1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240995F4

Cat no : 83830-2-RR

Synonyms

ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase

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Validation Data Gallery

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  WB analysis using 83830-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83830-2-RR (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical squamous cancer using 83830-2-RR

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83830-2-RR (ALDH1A1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83830-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83830-2-RR against Human ALDH1A1 were performed. The affinity constant is 0.322 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, mouse liver tissue, mouse lung tissue
• Positive IHC detected in: human cervical squamous cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83830-2-RR is ALDH1A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ALDH1A1 fusion protein Ag8665
• Full Name: aldehyde dehydrogenase 1 family, member A1
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC001505
• Gene Symbol: ALDH1A1
• Gene ID (NCBI): 216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).