AKAP8 Polyclonal antibody

AKAP8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31542-1-AP

Synonyms

A kinase anchor protein 8, A kinase anchor protein 95 kDa, AKAP 8, AKAP 95, AKAP8, AKAP95, DKFZp586B1222

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Validation Data Gallery

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  WB analysis using 31542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31542-1-AP (AKAP8 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HL-60 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31542-1-AP targets AKAP8 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKAP8 fusion protein Ag35816
• Full Name: A kinase (PRKA) anchor protein 8
• Calculated Molecular Weight: 76kDa，692aa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: NM_005858.3
• Gene Symbol: AKAP8
• Gene ID (NCBI): 10270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP8 (A-kinase anchor protein 8), also called AKAP95, is a A-kinase anchor protein that directs activity of protein kinase A by tethering the enzyme near its physiologic substrates (PMID: 9473338; 10601332). It is associated with chromatin and nuclear matrix which has a remarkable activity in promoting chromatin transcription (PMID: 31980632), and it is also required for cell cycle G2/M transition and histone deacetylation during mitosis (PMID: 16980585).