CoraLite® Plus 750-conjugated AKAP3 Recombinant antibody

AKAP3 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230278C3

Cat no : CL750-82986

Synonyms

---

Validation Data Gallery

• 

  WB analysis using CL750-82986

  Rat testis was subjected to SDS PAGE followed by western blot with CL750-82986 (AKAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82986 targets AKAP3 in WB applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AKAP3 fusion protein Ag33655
• Full Name: A kinase (PRKA) anchor protein 3
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC047535
• Gene Symbol: AKAP3
• Gene ID (NCBI): 10566
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.