AKAP12 Polyclonal antibody, PBS Only

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  WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 25199-1-AP (same clone as 25199-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 25199-1-AP (same clone as 25199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25199-1-AP (AKAP12 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AKAP12 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 25199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

25199-1-PBS targets AKAP12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKAP12 fusion protein Ag17769
• Full Name: A kinase (PRKA) anchor protein 12
• Calculated Molecular Weight: 1782 aa, 191 kDa
• Observed Molecular Weight: 200-300 kDa
• GenBank Accession Number: BC157067
• Gene Symbol: AKAP12
• Gene ID (NCBI): 9590
• RRID: AB_2879954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02952
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)