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AKAP12 Polyclonal antibody
AKAP12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 25199-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
25199-1-AP targets AKAP12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKAP12 fusion protein Ag17769 |
| Full Name | A kinase (PRKA) anchor protein 12 |
| Calculated Molecular Weight | 1782 aa, 191 kDa |
| Observed Molecular Weight | 200-300 kDa |
| GenBank Accession Number | BC157067 |
| Gene Symbol | AKAP12 |
| Gene ID (NCBI) | 9590 |
| RRID | AB_2879954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP12 antibody 25199-1-AP | Download protocol |
| IHC protocol for AKAP12 antibody 25199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Transl Med Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy | ||
Mol Ther Nucleic Acids The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p. | ||
Front Endocrinol (Lausanne) Construction of novel hypoxia-related gene model for prognosis and tumor microenvironment in endometrial carcinoma | ||
Sci Rep β-catenin regulates the formation of multiple nephron segments in the mouse kidney. | ||
Surgery Heat shock protein A12B gene therapy improves perfusion, promotes neovascularization, and decreases fibrosis in a murine model of hind limb ischemia. | ||
Am J Transl Res Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Pradeep (Verified Customer) (07-20-2020) | I used for immunofluorescence in mouse tissue sample. it is working great.
|
FH Rushita (Verified Customer) (11-26-2019) | The Ab works very well for detection of endogenous AKAP12 for western blotting (1:1000) in HEK 293 and COS7 cell lines.
|
