AIM2 Monoclonal antibody, PBS Only

AIM2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C6E1

Cat no : 66902-3-PBS

Synonyms

PYHIN4, Interferon-inducible protein AIM2, 4C6E1

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Validation Data Gallery

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  WB analysis using 66902-3-Ig (same clone as 66902-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66902-3-Ig (AIM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-3-PBS in a different storage buffer formulation.

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Product Information

66902-3-PBS targets AIM2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIM2 fusion protein Ag14607
• Full Name: absent in melanoma 2
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC010940
• Gene Symbol: AIM2
• Gene ID (NCBI): 9447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]