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AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C6E1

Cat no : 66902-3-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIM2 Monoclonal antibody (66902-3-Ig)

    WB analysis using 66902-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66902-3-Ig (AIM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Karpas-299 cells, U266 cells, MJ cells, HuT 78 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66902-3-Ig targets AIM2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607
Full Name absent in melanoma 2
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 66902-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols