AIM2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

AIM2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20590-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20590-1-AP (same clone as 20590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 20590-1-AP (same clone as 20590-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 20590-1-AP (same clone as 20590-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20590-1-AP (same clone as 20590-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20590-1-AP (same clone as 20590-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 20590-1-AP (same clone as 20590-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 20590-1-AP (same clone as 20590-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 20590-1-AP (same clone as 20590-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20590-1-AP (same clone as 20590-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 20590-1-AP (same clone as 20590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20590-1-AP (same clone as 20590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20590-1-PBS in a different storage buffer formulation.

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Product Information

20590-1-PBS targets AIM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AIM2 fusion protein Ag14520
• Full Name: absent in melanoma 2
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010940
• Gene Symbol: AIM2
• Gene ID (NCBI): 9447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].