AIM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AIM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 20590-1-AP

Synonyms

PYHIN4, Interferon-inducible protein AIM2

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Validation Data Gallery

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  WB analysis using 20590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20590-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20590-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20590-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 20590-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20590-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20590-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20590-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20590-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 20590-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 20590-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 20590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue, MCF-7 cells, A549 cells, COLO 320 cells, HepG2 cells, Raji cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 10 publications below

Product Information

20590-1-AP targets AIM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AIM2 fusion protein Ag14520
• Full Name: absent in melanoma 2
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC010940
• Gene Symbol: AIM2
• Gene ID (NCBI): 9447
• RRID: AB_10694420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.; Authors - Xiaolong He ; KD Validated
mouse	WB	Mucosal Immunol; AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection.; Authors - G-Q Hu
human	IHC	Front Immunol; Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.; Authors - Qingling Guo
human	WB	Front Cell Dev Biol; Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.; Authors - Shuai Li ; KD Validated
human	WB,IHC	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu
human	WB	Front Oncol; Identification of pyroptosis-related lncRNA signature and AC005253.1 as a pyroptosis-related oncogene in prostate cancer; Authors - JiangFan Yu

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