AIF Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

AIF Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, Canine

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C12B2

Cat no : 67791-1-PBS

Synonyms

AIF, AIFM1, Cell and organelle markers, PDCD8

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Validation Data Gallery

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  WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

  WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67791-1-PBS targets AIF in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Canine samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIF fusion protein Ag12400
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 1
• Calculated Molecular Weight: 609 aa, 66 kDa
• Observed Molecular Weight: 67 kDa, 57 kDa
• GenBank Accession Number: BC111065
• Gene Symbol: AIFM1
• Gene ID (NCBI): 9131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).