AHNAK2 Polyclonal antibody

AHNAK2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27078-1-AP

Synonyms

AHNAK nucleoprotein 2, AHNAK2, C14orf78, KIAA2019, Protein AHNAK2

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Validation Data Gallery

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  WB analysis of HeLa using 27078-1-AP

  HeLa cells were subjected to Tris-acetate gel system followed by western blot with 27078-1-AP (AHNAK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 27078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27078-1-AP (AHNAK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 27078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27078-1-AP (AHNAK2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27078-1-AP targets AHNAK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHNAK2 fusion protein Ag25895
• Full Name: AHNAK nucleoprotein 2
• Calculated Molecular Weight: 617 kDa
• GenBank Accession Number: BC049216
• Gene Symbol: AHNAK2
• Gene ID (NCBI): 113146
• RRID: AB_2880744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.