AGO2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

AGO2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2H12

Cat no : 67934-1-PBS

Synonyms

EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2

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Validation Data Gallery

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  WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67934-1-Ig (same clone as 67934-1-PBS)

  WB result of AGO2 antibody (67934-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGO2 transfected HepG2 cells. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67934-1-Ig (same clone as 67934-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67934-1-Ig (same clone as 67934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67934-1-Ig (same clone as 67934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67934-1-Ig (same clone as 67934-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AGO2 Monoclonal antibody (67934-1-Ig, Clone:1G2H12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

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Product Information

67934-1-PBS targets AGO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AGO2 fusion protein Ag27772
• Full Name: eukaryotic translation initiation factor 2C, 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC007633
• Gene Symbol: EIF2C2
• Gene ID (NCBI): 27161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.