AGO2 Monoclonal antibody

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• KD/KO Validated

AGO2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Publications

43

CloneNo.

1G2H12

Cat no : 67934-1-Ig

Synonyms

EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2

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Validation Data Gallery

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  WB analysis using 67934-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 67934-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HepG2 using 67934-1-Ig

  WB result of AGO2 antibody (67934-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGO2 transfected HepG2 cells.

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  WB analysis of 4T1 using 67934-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 67934-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67934-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 67934-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AGO2 Monoclonal antibody (67934-1-Ig, Clone:1G2H12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: LNCaP cells, K-562 cells, 4T1 cells, HepG2 cells, HeLa cells, HEK-293 cells, MCF-7 cells, HSC-T6 cells
• Positive IHC detected in: human pancreas cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 5 publications below
• RIP: See 30 publications below

Product Information

67934-1-Ig targets AGO2 in WB, IHC, IF, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AGO2 fusion protein Ag27772
• Full Name: eukaryotic translation initiation factor 2C, 2
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC007633
• Gene Symbol: EIF2C2
• Gene ID (NCBI): 27161
• RRID: AB_2918686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.

Publications

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
mouse	RIP	Environ Health Perspect; Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study.; Authors - Bingwei Yang
human	WB,IP	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
mouse	RIP	Life Sci; LncRNA PEG11as aggravates cerebral ischemia/reperfusion injury after ischemic stroke through miR-342-5p/PFN1 axis; Authors - Zhijun Yu
mouse	WB	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	RIP	Cancer Sci; ELF1 suppresses autophagy to reduce cisplatin resistance via the miR-152-3p/NCAM1/ERK axis in lung cancer cells; Authors - Lifeng Zhao