AGER/RAGE Recombinant antibody

AGER/RAGE Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240706C3

Cat no : 83759-5-RR

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706C3

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Validation Data Gallery

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  WB analysis using 83759-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83759-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 83759-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 83759-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83759-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83759-5-RR against Rat AGER/RAGE were performed. The affinity constant is 3.46 nM.

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse heart tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83759-5-RR is AGER/RAGE Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: advanced glycosylation end product-specific receptor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• Gene Symbol: Ager
• Gene ID (NCBI): 81722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).