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Rat AGER/RAGE Recombinant antibody, PBS Only

AGER/RAGE Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240706C3

Cat no : 83759-5-PBS

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706C3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGER/RAGE Recombinant antibody (83759-5-RR)

    WB analysis using 83759-5-RR (same clone as 83759-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83759-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83759-5-RR

    Affinity and Kinetic Characterization of 83759-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-5-RR against Rat AGER/RAGE were performed. The affinity constant is 3.46 nM.

Product Information

The immunogen of 83759-5-PBS is AGER/RAGE Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name advanced glycosylation end product-specific receptor
Calculated Molecular Weight43 kDa
Observed Molecular Weight43 kDa
Gene Symbol Ager
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).