AES Recombinant antibody

AES Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240987D9

Cat no : 83878-5-RR

Synonyms

TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2

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Validation Data Gallery

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  WB analysis using 83878-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83878-5-RR (AES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83878-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83878-5-RR against Human AES were performed. The affinity constant is 1.76 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, DU 145 cells, MDA-MB-231 cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83878-5-RR is AES Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AES fusion protein Ag21021
• Full Name: amino-terminal enhancer of split
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 22-29 kDa
• GenBank Accession Number: BC113735
• Gene Symbol: AES
• Gene ID (NCBI): 166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).