AES Recombinant antibody, PBS Only

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  WB analysis using 83878-5-RR (same clone as 83878-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83878-5-RR (AES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83878-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83878-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83878-5-RR against Human AES were performed. The affinity constant is 1.76 nM.

AES Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83878-5-PBS

Clone No.240987D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2

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Product Information

83878-5-PBS targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AES fusion protein Ag21021
• Full Name: amino-terminal enhancer of split
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 22-29 kDa
• GenBank Accession Number: BC113735
• Gene Symbol: AES
• Gene ID (NCBI): 166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q08117
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).