CoraLite®594-conjugated ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F7A5

Cat no : CL594-67067

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

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Validation Data Gallery

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  IF Staining of Jurkat using CL594-67067

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 ADRBK1 antibody (CL594-67067, Clone: 2F7A5 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-67067

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ADRBK1 (CL594-67067, Clone:2F7A5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67067 targets ADRBK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADRBK1 fusion protein Ag5071
• Full Name: adrenergic, beta, receptor kinase 1
• Calculated Molecular Weight: 689 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC037963
• Gene Symbol: ADRBK1
• Gene ID (NCBI): 156
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.