ADK Monoclonal antibody

ADK Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1G1A5

Cat no : 66929-1-Ig

Synonyms

EC:2.7.1.20, AK, adenosine kinase, Adenosine 5'-phosphotransferase, 1G1A5

---

Validation Data Gallery

• 

  WB analysis using 66929-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66929-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66929-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66929-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66929-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of NIH/3T3 using 66929-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue, human breast cancer tissue
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

66929-1-Ig targets ADK in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADK fusion protein Ag7466
• Full Name: adenosine kinase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC003568
• Gene Symbol: ADK
• Gene ID (NCBI): 132
• RRID: AB_2882255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles
human,mouse	WB,IHC	Gastroenterology; Hepatocyte Adenosine Kinase Promotes Excessive Fat Deposition and Liver Inflammation; Authors - Honggui Li
mouse	WB	EMBO J; Regulation of LRRK2 mRNA stability by ATIC and its substrate AICAR through ARE-mediated mRNA decay in Parkinson's disease; Authors - Qinfang Liu