Adiponectin receptor 1 Monoclonal antibody

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• KD/KO Validated

Adiponectin receptor 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1F12B3

Cat no : 66619-1-Ig

Synonyms

ACDCR1, Adiponectin receptor 1, Adiponectin receptor protein 1, ADIPOR1, CGI 45, CGI45, PAQR1, TESBP1A

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Validation Data Gallery

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  WB analysis of HEK-293 using 66619-1-Ig

  WB result of Adiponectin receptor 1 antibody (66619-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Adiponectin receptor 1 transfected HEK-293 cells.

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  WB analysis of HeLa using 66619-1-Ig

  HeLa cells, L02 cells, and HepG2 cells were subjected to SDS PAGE followed by western blot with 66619-1-Ig (Adiponectin receptor 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

66619-1-Ig targets Adiponectin receptor 1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Adiponectin receptor 1 fusion protein Ag18187
• Full Name: adiponectin receptor 1
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC010743
• Gene Symbol: Adiponectin receptor 1
• Gene ID (NCBI): 51094
• RRID: AB_2881979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin have been identified: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). They are seven-transmembrane domain receptors and mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is found in many tissues, being particularly abundant in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 24417942). AdipoR1 has a high affinity for globular adiponectin and a very low affinity for full-length adiponectin. AdipoR2 has an intermediate affinity for both forms. The human ADIPOR1 gene maps to chromosome 1q32.1, and encodes a 375-amino acid protein with a molecular weight of 43 kDa. This antibody was raised against 1-72aa of human AdipoR1.

Publications

Species	Application	Title
mouse	WB	Food Funct; A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice.; Authors - Xin Li
mouse	WB	Food Funct; Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.; Authors - Xiaoling Chen
human	WB	Biol Open; A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference.; Authors - Nina Graffmann
pig	WB	Anim Biotechnol; Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.; Authors - Lu Xiang
pig	WB	Anim Biotechnol; Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets; Authors - Xiaoling Chen
mouse	WB	J Agric Food Chem; Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway; Authors - Chao Tang