Adiponectin Recombinant antibody

Adiponectin Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241082E1

Cat no : 83961-3-RR

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

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Validation Data Gallery

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  IHC staining of mouse brown adipose using 83961-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 83961-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of 3T3-L1 using 83961-3-RR

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using ADIPOQ antibody (83961-3-RR, Clone: 241082E1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83961-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83961-3-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 3T3-L1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83961-3-RR is Adiponectin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Adiponectin fusion protein Ag16304
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 244 aa, 26 kDa
• GenBank Accession Number: BC096308
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 9370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.