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Adiponectin Recombinant antibody, PBS Only (Detector)

Adiponectin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241082E1

Cat no : 83961-3-PBS

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Recombinant antibody (83961-3-RR)

    IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Recombinant antibody (83961-3-RR)

    IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using Adiponectin Recombinant antibody (83961-3-RR)

    IF Staining of 3T3-L1 using 83961-3-RR (same clone as 83961-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using ADIPOQ antibody (83961-3-RR, Clone: 241082E1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00919-2

    Cytometric bead array standard curve of MP00919-2

    Cytometric bead array standard curve of MP00919-2, Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83961-1-PBS. Detection antibody: 83961-3-PBS. Standard: Ag16304. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83961-3-RR

    Affinity and Kinetic Characterization of 83961-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83961-3-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.

Product Information

83961-3-PBS targets Adiponectin as part of a matched antibody pair:

MP00919-2: 83961-1-PBS capture and 83961-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Adiponectin fusion protein Ag16304
Full Name adiponectin, C1Q and collagen domain containing
Calculated Molecular Weight 244 aa, 26 kDa
GenBank Accession NumberBC096308
Gene Symbol Adiponectin
Gene ID (NCBI) 9370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.