ADH1C Recombinant antibody

ADH1C Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241623A3

Cat no : 84546-2-RR

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 84546-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84546-2-RR (ADH1C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse liver tissue, mouse colon tissue, human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84546-2-RR is ADH1C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADH1C fusion protein Ag13528
• Full Name: alcohol dehydrogenase 1C (class I), gamma polypeptide
• Calculated Molecular Weight: 375 aa, 40 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC062476
• Gene Symbol: ADH1C
• Gene ID (NCBI): 126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alcohol dehydrogenase 1C(ADH1C), also known as ADH3, is an alcohol dehydrogenase that can catalyze ethanol oxidation to metabolite acetaldehyde. According to THE HUMAN PROTEIN ATLAS database, ADH1C locates mainly to the cytosol and plasma membrane. Genetic polymorphism of the ADH1C gene (ADH1C*1 allele) is associated with various human cancers, such as gastric cancer, oral cancer and CRC, for it encodes an alcohol dehydrogenase producing 2.5 times more acetaldehyde.(PMID: 35300114)