• Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F1B4

Cat no : CL488-66939

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125



Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66939 targets ADH1B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADH1B fusion protein Ag10630
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC033009
Gene Symbol ADH1B
Gene ID (NCBI) 125
RRIDAB_2919392
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths488 nm / 515 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADH1B antibody CL488-66939Download protocol
Standard Protocols
Click here to view our Standard Protocols