CoraLite®594-conjugated ADC Monoclonal antibody

ADC Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A2B11

Cat no : CL594-67884

Synonyms

AZIN2, AzI2, Arginine decarboxylase, ARGDC, Antizyme inhibitor 2

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Validation Data Gallery

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  IF Staining of A431 using CL594-67884

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 ADC antibody (CL594-67884, Clone: 1A2B11 ) at dilution of 1:200.

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  FC experiment of A431 using CL594-67884

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ADC (CL594-67884, Clone:1A2B11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67884 targets ADC in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADC fusion protein Ag31049
• Full Name: arginine decarboxylase
• Calculated Molecular Weight: 460 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC028128
• Gene Symbol: ADC
• Gene ID (NCBI): 113451
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Antizyme inhibitor 2 (AZIN2) is a member of the antizyme inhibitor family, witch is able to bind to AZs and inhibit their action on both ODC activity and polyamine uptake. AZIN2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of azin2 has also been observed in brains of patients with Alzheimer's disease (PMID: 19832840) . AZIN2 has 5 described insoforms produced by alternative splicing. The molecular weight of AZIN2 is about 50 kDa.