ADAR1 Recombinant antibody

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  WB analysis using 85009-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85009-1-RR (ADAR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ADAR1 Recombinant Antibody for WB, ELISA

Cat No. 85009-1-RR

Clone No.241595E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ADAR, DRADA, Double-stranded RNA-specific adenosine deaminase, adenosine deaminase RNA specific, 241595E8

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85009-1-RR targets ADAR1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAR1 fusion protein Ag5609
• Full Name: adenosine deaminase, RNA-specific
• Calculated Molecular Weight: 136 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC038227
• Gene Symbol: ADAR1
• Gene ID (NCBI): 103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55265
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).