ADAMTS4 Recombinant antibody

ADAMTS4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4F9

Cat no : 82744-2-RR

Synonyms

ADAM-TS 4, ADAMTS 4, ADAM TS4, ADAM TS 4, A disintegrin and metalloproteinase with thrombospondin motifs 4

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Validation Data Gallery

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  WB analysis using 82744-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82744-2-RR (ADAMTS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 82744-2-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ADAMTS4 Recombinant antibody (82744-2-RR, Clone:4F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82744-2-RR against Human ADAMTS4 were performed. The affinity constant is 0.632 nM.

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Tested Applications

• Positive WB detected in: THP-1 cells, HEK-293T cells, SH-SY5Y cells, Caco-2 cells, mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82744-2-RR targets ADAMTS4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAMTS4 fusion protein Ag2443
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
• Calculated Molecular Weight: 837 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC030812
• Gene Symbol: ADAMTS4
• Gene ID (NCBI): 9507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).