ADAMTS2 Recombinant antibody

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  WB analysis using 84476-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84476-6-RR (ADAMTS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84476-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ADAMTS2 antibody (84476-6-RR, Clone: 241860B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84476-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84476-6-RR against Human ADAMTS2 were performed. The affinity constant is 0.472 nM.

ADAMTS2 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84476-6-RR

Clone No.241860B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2

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Tested Applications

• Positive WB detected in: A431 cells, COLO 205 cells, PC-3 cells, HT-1080 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84476-6-RR targets ADAMTS2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAMTS2 fusion protein Ag34957
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 2
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 110~130 kDa
• GenBank Accession Number: NM_014244
• Gene Symbol: ADAMTS2
• Gene ID (NCBI): 9509
• RRID: AB_3671997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O95450
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).