WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)
Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 67870-1-Ig (same clone as 67870-1-PBS)
WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 67870-1-Ig (same clone as 67870-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67870-1-Ig (same clone as 67870-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of rat spleen using 67870-1-Ig (same clone as 67870-1-PBS)
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of rabbit spleen using 67870-1-Ig (same clone as 67870-1-PBS)
rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
FC experiment of U2OS using 67870-1-Ig (same clone as 67870-1-PBS)
1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)
Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 67870-1-Ig (same clone as 67870-1-PBS)
WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 67870-1-Ig (same clone as 67870-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67870-1-Ig (same clone as 67870-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of rat spleen using 67870-1-Ig (same clone as 67870-1-PBS)
rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis of rabbit spleen using 67870-1-Ig (same clone as 67870-1-PBS)
rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of U2OS using 67870-1-Ig (same clone as 67870-1-PBS)
1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
ADA Monoclonal antibody
Catalog Number
67870-1-PBS
Citations
-
Dilutions
Applications
WB, FC (Intra), ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
