ADA Monoclonal antibody

• Featured Product
• KD/KO Validated

ADA Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, pig, rabbit, mouse

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2C4E9

Reviews (1)

Cat no : 67870-1-Ig

Synonyms

EC:3.5.4.4, ADA1, 2C4E9

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Validation Data Gallery

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  WB analysis using 67870-1-Ig

  Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67870-1-Ig

  WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells.

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  WB analysis of rabbit spleen using 67870-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67870-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 67870-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67870-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67870-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U2OS using 67870-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, LNCaP cells, HEK-293 cells, Sp2/0 cells, rat spleen tissue, rabbit spleen tissue, MOLT-4 cells, pig spleen tissue
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67870-1-Ig targets ADA in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat, pig, rabbit, mouse samples.

• Tested Reactivity: Human, rat, pig, rabbit, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADA fusion protein Ag31326
• Full Name: adenosine deaminase
• Calculated Molecular Weight: 363 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC040226
• Gene Symbol: ADA
• Gene ID (NCBI): 100
• RRID: AB_2918628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-30-2024)

Works ok on WB with mouse liver. The signal is weak. It is better to use a dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Moues liver

ADA Antibody Western Blot validation (1:4000 dilution) in Moues liver (Cat no:67870-1-Ig)