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CoraLite® Plus 488-conjugated ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12C6

Cat no : CL488-68037

Synonyms

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B, Actin-related protein 3B, Actin-related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP3-beta, ARP4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated ACTR3B Monoclonal an (CL488-68037)

    FC experiment of Jurkat using CL488-68037

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACTR3B (CL488-68037, Clone:1G12C6) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68037 targets ACTR3B in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACTR3B fusion protein Ag10218
Full Name ARP3 actin-related protein 3 homolog B (yeast)
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC008682
Gene Symbol ACTR3B
Gene ID (NCBI) 57180
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ACTR3B antibody CL488-68037Download protocol
Standard Protocols
Click here to view our Standard Protocols