CoraLite® Plus 488-conjugated ACTN3 Polyclonal antibody

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  IF Staining of mouse skeletal muscle using CL488-24378

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CoraLite® Plus 488 ACTN3 antibody (CL488-24378) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of C2C12 using CL488-24378

  1x10^6 C2C12 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Actn3 Polyclonal Antibody (CL488-24378)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ACTN3 Polyclonal Antibody for IF-P, FC (Intra)

Cat No. CL488-24378

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

F-actin cross-linking protein, Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, Alpha actinin 3, ACTN 3

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Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24378 targets ACTN3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTN3 fusion protein Ag19479
• Full Name: actinin, alpha 3
• Calculated Molecular Weight: 901 aa, 103 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC099647
• Gene Symbol: ACTN3
• Gene ID (NCBI): 89
• RRID: AB_3672764
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08043
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.