ACTC1-specific Monoclonal antibody, PBS Only

ACTC1-specific Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-PBS

Synonyms

ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1

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Validation Data Gallery

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  WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

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Product Information

66125-1-PBS targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha, cardiac muscle 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_005159
• Gene Symbol: ACTC1
• Gene ID (NCBI): 70
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.