ACTA1-Specific Recombinant antibody, PBS Only

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  WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

ACTA1-Specific Uni-rAb^TM Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 82714-5-PBS

Clone No.5B15

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IF-P, Indirect ELISA

Synonyms

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

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Product Information

82714-5-PBS targets ACTA1-Specific in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 1, skeletal muscle
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: NM_001100
• Gene Symbol: ACTA1
• Gene ID (NCBI): 58
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68133
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.