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ACTA1-Specific Recombinant antibody

ACTA1-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig, chicken, human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5B15

Cat no : 82714-5-RR

Synonyms

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACTA1-Specific Recombinant antibody (82714-5-RR)

    WB analysis using 82714-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using ACTA1-Specific Recombinant antibody (82714-5-RR)

    IF Staining of mouse skeletal muscle using 82714-5-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, chicken skeletal muscle tissue
Positive IF/ICC detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82714-5-RR targets ACTA1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat, pig, chicken, human samples.

Tested Reactivity mouse, rat, pig, chicken, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name actin, alpha 1, skeletal muscle
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberNM_001100
Gene Symbol ACTA1
Gene ID (NCBI) 58
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

Protocols

Product Specific Protocols
WB protocol for ACTA1-Specific antibody 82714-5-RRDownload protocol
IF protocol for ACTA1-Specific antibody 82714-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols