ACTA1-Specific Polyclonal antibody, PBS Only

ACTA1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 17521-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 17521-1-AP (ACTA1-Specific Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hysteromyoma using 17521-1-AP (same clone as 17521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17521-1-AP (ACTA1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 17521-1-AP (same clone as 17521-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTA1-Specific (17521-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17521-1-PBS in a different storage buffer formulation.

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Product Information

17521-1-PBS targets ACTA1-Specific in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 1, skeletal muscle
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: NM_001100
• Gene Symbol: ACTA1
• Gene ID (NCBI): 58
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.