ACTA1-Specific Polyclonal antibody

ACTA1-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17521-1-AP

Synonyms

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, ACTA1 skeletal Muscle Actin Specific, ACTA1-Specific, Actin, alpha skeletal muscle, Alpha actin 1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, sarcomeric actin



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human hysteromyoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha 1, skeletal muscle
Calculated molecular weight 42 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberNM_001100
Gene symbol ACTA1
Gene ID (NCBI) 58
RRIDAB_2273608
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

Protocols

Product Specific Protocols
WB protocol for ACTA1-Specific antibody 17521-1-APDownload protocol
IHC protocol for ACTA1-Specific antibody 17521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Clin Invest

Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.

Authors - Andres Ramirez-Martinez
humanWB

Cancer Lett

Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop.

Authors - Yue Zhang
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
humanWB

Int J Mol Sci

Neddylation Regulates Class IIa and III Histone Deacetylases to Mediate Myoblast Differentiation

Authors - Hongyi Zhou
ratIF,WB

Aging (Albany NY)

Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.

Authors - Xin Zhang
mouseWB

iScience

A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart

Authors - Aude Angelini