ACSL4/FACL4 Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

ACSL4/FACL4 Uni-rAb^TM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3I9

Cat no : 81196-1-PBS

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Validation Data Gallery

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  WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

  WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

  IP result of anti-ACSL4/FACL4 (IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

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Product Information

81196-1-PBS targets ACSL4/FACL4 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACSL4/FACL4 fusion protein Ag18085
• Full Name: acyl-CoA synthetase long-chain family member 4
• Calculated Molecular Weight: 711 aa, 79 kDa
• Observed Molecular Weight: 70 kDa, 75 kDa
• GenBank Accession Number: BC034959
• Gene Symbol: ACSL4
• Gene ID (NCBI): 2182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).