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ACSL4/FACL4 Recombinant antibody
ACSL4/FACL4 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3I9
Cat no : 81196-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, U-87 MG cells, HT-29 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human liver cancer tissue, human stomach cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
81196-1-RR targets ACSL4/FACL4 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ACSL4/FACL4 fusion protein Ag18085 |
Full Name | acyl-CoA synthetase long-chain family member 4 |
Calculated Molecular Weight | 711 aa, 79 kDa |
Observed Molecular Weight | 70 kDa, 75 kDa |
GenBank Accession Number | BC034959 |
Gene Symbol | ACSL4 |
Gene ID (NCBI) | 2182 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACSL4/FACL4 antibody 81196-1-RR | Download protocol |
IHC protocol for ACSL4/FACL4 antibody 81196-1-RR | Download protocol |
IP protocol for ACSL4/FACL4 antibody 81196-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Pharmacol Epigallocatechin gallate alleviates high-fat diet-induced hepatic lipotoxicity by targeting mitochondrial ROS-mediated ferroptosis | ||
BMC Cancer Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells | ||
MedComm (2020) Novel aspect of neprilysin in kidney fibrosis via ACSL4-mediated ferroptosis of tubular epithelial cells |