• Featured Product
  • KD/KO Validated

ACSL4/FACL4 Recombinant antibody

ACSL4/FACL4 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3I9

Cat no : 81196-1-RR

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, U-87 MG cells, HT-29 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81196-1-RR targets ACSL4/FACL4 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACSL4/FACL4 fusion protein Ag18085
Full Name acyl-CoA synthetase long-chain family member 4
Calculated Molecular Weight 711 aa, 79 kDa
Observed Molecular Weight70 kDa, 75 kDa
GenBank Accession NumberBC034959
Gene Symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).

Protocols

Product Specific Protocols
WB protocol for ACSL4/FACL4 antibody 81196-1-RRDownload protocol
IHC protocol for ACSL4/FACL4 antibody 81196-1-RRDownload protocol
IP protocol for ACSL4/FACL4 antibody 81196-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Front Pharmacol

Epigallocatechin gallate alleviates high-fat diet-induced hepatic lipotoxicity by targeting mitochondrial ROS-mediated ferroptosis

Authors - Shi-Bin Ding
humanWB

BMC Cancer

Oridonin-induced ferroptosis and apoptosis: a dual approach to suppress the growth of osteosarcoma cells

Authors - Feifan Zhang
humanIHC,WB

MedComm (2020)

Novel aspect of neprilysin in kidney fibrosis via ACSL4-mediated ferroptosis of tubular epithelial cells

Authors - Weijing Lai