CoraLite® Plus 488-conjugated ACOX1 Monoclonal antibody

ACOX1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E12E10

Cat no : CL488-68017

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

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Validation Data Gallery

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  FC experiment of NIH/3T3 using CL488-68017

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ACOX1 (CL488-68017, Clone:3E12E10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68017 targets ACOX1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACOX1 fusion protein Ag28037
• Full Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC010425
• Gene Symbol: ACOX1
• Gene ID (NCBI): 51
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C