ACOT7 Monoclonal antibody, PBS Only

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  WB analysis using 68140-1-Ig (same clone as 68140-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68140-1-Ig (ACOT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68140-1-PBS in a different storage buffer formulation.

ACOT7 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68140-1-PBS

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse, Rabbit, Pig

Applications

WB, Indirect ELISA

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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Product Information

68140-1-PBS targets ACOT7 in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACOT7 fusion protein Ag8975
• Full Name: acyl-CoA thioesterase 7
• Calculated Molecular Weight: 370 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC017365
• Gene Symbol: ACOT7
• Gene ID (NCBI): 11332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00154
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824).