ACOT7 Monoclonal antibody

ACOT7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9C7

Cat no : 68140-1-Ig

Synonyms

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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Validation Data Gallery

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  WB analysis using 68140-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68140-1-Ig (ACOT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68140-1-Ig targets ACOT7 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit, Pig samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACOT7 fusion protein Ag8975
• Full Name: acyl-CoA thioesterase 7
• Calculated Molecular Weight: 370 aa, 40 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC017365
• Gene Symbol: ACOT7
• Gene ID (NCBI): 11332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterases (ACOTs) are enzymes that catalyze hydrolysis of fatty acyl-CoAs to free fatty acids and CoA-SH. ACOTs are expressed ubiquitously in prokaryotes and eukaryotes (PMID: 11755680). ACOT7 also known as BACH, CTE-II, ACT, ACH1, BACHa, is one of the most extensively studied ACOTs as a major isoform of the ACOT family (PMID: 17563367). The enzyme is localized to the cytosol and is highly expressed in brain tissue and testis tissue, showing a preference for long-chain acyl-CoA substrates with fatty acid chains of 8-16 carbon atoms (C8-C16) (PMID: 28518146). It has been identified that ACOT7 plays a role in neurotoxic prevention and is a candidate drug target in inflammatory disease (PMID: 17563367, 23459938, 10509865). ACOT7 has 7 isoforms as molecular between 27-40 kDa, it may also form dimer (~70 kDa) (PMID: 20470824).