CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody

ACAA1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12319

Synonyms

Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal

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Validation Data Gallery

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  IF Staining of U-251 using CL488-12319

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 ACAA1 antibody (CL488-12319) at dilution of 1:200.

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  FC experiment of U-251 using CL488-12319

  1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody (CL488-12319)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12319 targets ACAA1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAA1 fusion protein Ag2972
• Full Name: acetyl-Coenzyme A acyltransferase 1
• Calculated Molecular Weight: 424 aa, 44 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC011977
• Gene Symbol: ACAA1
• Gene ID (NCBI): 30
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).