CoraLite®594-conjugated ABCD1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ABCD1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H8E5

Cat no : CL594-60153

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

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Validation Data Gallery

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  IF Staining of HeLa using CL594-60153

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated ABCD1 antibody (CL594-60153, Clone: 1H8E5 ) at dilution of 1:100. Green: Stain with CoraLite®488-Phalloidin (CatNo. PF00001).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60153 targets ABCD1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCD1 fusion protein Ag12757
• Full Name: ATP-binding cassette, sub-family D (ALD), member 1
• Calculated Molecular Weight: 745 aa, 83 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC025358
• Gene Symbol: ABCD1
• Gene ID (NCBI): 215
• RRID: AB_2919897
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.